Application Note ► Purifying common light-chain bispecific antibodies using MCSGP
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چکیده
In this application note the purification of a common light-chain bispecific antibody using Multicolumn countercurrent solvent gradient purification (MCSGP) is described. The KNAUER Contichrom® system that is offered in cooperation of KNAUER and Chromacon is the only system on the market that can be used in MCSGP mode for difficult bioseparations. MCSGP is a countercurrent chromatographic process that is particularly suited for applications in the field of bioseparations. MCSGP is suitable for three-fraction chromatographic separations and able to perform linear gradients. It is superior to batch chromatography in terms of buffer consumption, yield, purity and productivity due to the countercurrent movement of the liquid and the solid phase.
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